Following is the report from Dr. Theodore D. Kessis, former faculty and employee of Johns Hopkins University Departments of Immunology and Infectious Diseases, Molecular Microbiology and Immunology and Pathology, and currently principal of Applied DNA Resources, regarding the blood and DNA evidence collected from the scene of the crime. The report is enlightening and casts much more than reasonable doubt upon the entire case.
September 2, 2006
Report of Findings
State of Nebraska vs. Herman D. Buckman
This report is being submitted in my capacity as an expert on behalf of Herman D. Buckman in connection with his appeal in State of Nebraska vs. Herman D. Buckman.
Cindy Riekenberg, coordinator at Advocates for Justice, Inc., requested my review of the Serologic and DNA testing performed by the University of Nebraska Medical Center (UNMC) and the State of Nebraska Criminalistics Laboratory in connection with this case. Ultimately, Ms. Riekenberg asked that I provide her with an opinion regarding the significance of analysis performed by UNMC in relationship to the previous testing performed by the State and testified to by it's expert.
I. Qualifications
I am a former faculty member, employee and student of the Johns Hopkins University, Departments of Immunology and Infectious Diseases, Molecular Microbiology and Immunology and Pathology. Currently, I serve as principal of Applied DNA Resources.
Over the past 22 years I have had the opportunity to design, utilize and review a wide range of DNA typing technologies utilized in the research, medical and forensic communities.
In conjunction with my former and present positions, I have extracted the DNA from thousands of specimens and performed an equal number of Polymerase Chain Reaction (PCR) procedures. In addition, I am familiar with the electrophoretic assays associated with the detection steps of such tests, having utilized and witnessed these procedures on numerous occasions. I am also familiar with the interpretation of data generated in association with such methodologies, having reviewed the testing and case file information associated with more than 225 cases involving forensic DNA testing.
In addition, I have qualified more than 35 times at both the State and Federal levels as an expert in the use and application of DNA typing, notably the use of PCR based detection of Short Tandem Repeats (STR).
II. Documents Examined
Ms. Riekenberg provided me with the following items for review:
- State of Nebraska Criminalistics Laboratory report date September 27, 1988
- UNMC report dated April 30, 2002
III. Findings
A review of the reports listed above indicate that in September of 1988, the State's laboratory reported the finding of blood on several key items of evidence. Of specific interest here, the lab reported a result for the identification of human blood (blood group subtype specific) on the following items:
- Sweater (item #56)
- Sleeve of a black leather jacket (item #57)
- Slippers (item #70)
In contrast to these finds, a report issued by the UNMC laboratory on April 30, 2002 indicates that on reexamination, no blood was detected on any of these items. It should be noted here that this report indicates that UNMC tested many more specific areas on these items than originally analyzed by the State's lab. Remarkably, UNMC went on to test samples taken from these items for the presence of DNA obtaining negative reults for all samples tested.
Such discordance between the State's original testing and that reported by UNMC is alarming given the inability of UNMC to replicate the detection of any blood, despite UNBC testing of many more samples than originally analyzed. I also found it surprising that UNMC failed to detect any DNA on these items given the State's report indicating that they had detected and typed blood on these items. Given the sensitivity of DNA analysis, such a grossly negative result is highly unusual given the number of items on which blood was claimed to have been found.
In addition to analyzing the items of evidence discussed above, UNMC also tested various samples derived from a cigarette butt alleged to have belonged to or have been used by the defendant (UNMC #02-359-4B, paper and filter; and 02-359-4C, paper only).
Testing by the lab revealed a mixture consistent with the contribution of DNA from two individuals, given that major and minor profiles were discernable in the samples. In addition, UNMC's report indicated that the partial profile detected on the cigarette paper (02-359-4C) was consistent with that possessed by the victim, Denise Stawkowski, indicating that she could not be excluded as a contributor.
With regard to sample 02-359-4B (cigarette paper and filter), the lab reported that the results were inconclusive with respect to any comparison to a number of reference sample tested at the same time, including those from the victim and defendant.
While I agree with UNMC's reported conclusion concerning the sample of cigarette paper, a closer examination of the results for sample #02-359-4B (cigarette paper and filter) clearly indicate that Mr. Buckman is excluded as a contributor of DNA. I base this conclusion on the fact that the lab observed: i) major and minor contributor to this sample, a result consistent with the presence of two individuals, and ii) alleles foreign to the victim and the defendant, thus excluding Mr. Buckman.
Specifically, the profile detected at the D3S1358 loci indicates the presence of three alleles (14, 17 and 18). Of key importance here is the fact that the 14 allele is foreign to both the victim and the defendant. And while Mr. Buckman does posses a 17 allele and the victim an 18 allele, the leab reports that the 14 and 17 alleles are more intense than 18 indicating that these two alleles more than likely belong to an individual other than Mr. Buckman, again a result in support of his exclusion as a contributor.
Further, if in the alternative, one were to argue that Mr. Buckman is still included as a contributor by virtue of "his" 17 allele, they would have to make two highly unlikely assumptions; i) the detected 14 allele was contributed by a third person and ii) the absence of Mr. Buckman's 16 allele merely reflects a failure of the test to detect it. Again, given that the lab reports the victim to be a contributor to the paper portion of the cigarette butt, a third party would have to be the contributor of the 14 allele detected in this sample.
Examination of the alleles detected at additional loci tested by the lab reveals a similar circumstance. Given that the victim has indisputably been detected as a contributor of DNA to this cigarette, Mr. Buckman is for all purposes excluded as a contributor. The only scenario that would include him as a contributor of DNA to this item would necessitate the contributions of DNA from three or more individuals. The data however does not support such a view. While such a circumstance is a formal possibility, the more reasonable interpretation of the data would conclude that two individuals contributed DNA to the cigarette, the victim and an unidentified male.
Summary
It is my opinion that the serologic testing previously performed by the State was neither accurate nor reliable and lacking in provative value. I base my opinion on the highly sensitive tests performed by UNMC on samples from this case. Given their results, it is also my opinion that any prior reporting of blood on any of the items described herein, would have been highly prejudicial in nature if presented toa Jury.
With respect to the cigarette butt, it is my opinion that any allegation ascribing either ownership or use of this evidentiary item to Mr. Buckman would also have been highly prejudicial if presented to a Jury. Further, it is my opinion that the results obtained by UNMC for the cigarette butt are exculpatory in nature, since they clearly exclued Mr. Buckman and point to the presence of an unidentified male in connection with this evidentiary item.
(Signed)
Dr. Theodore D. Kessis
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